Welcome Guest
Sign In
Register
Products
Popular Categories
Antibody Search
ELISA Kit Search
Biomolecules
Flow Cytometers
Thermal Cyclers
Microscopes
Transfection Reagents
Featured Products
New Products
Articles
Resources
eBooks
Bench Tips
Tech Insights
Editorial Articles
News
Product Reviews
Future Lab
Multimedia
Biocompare Webinars
Immunotherapy
Reproducibility
Transformative Technologies
Podcasts
Tech Talks
Bench Tip Videos
All Videos and Webinars
Life Science Webinars
Write a Review
Antibodies
Kits/Reagents
Equipment
Services
Resources
Promotions
Events
AACR SPOTLIGHT
Register
Sign In
Biocompare
Products
Cloning and Expression
Enzymes
AflIII
Supplier Images
AflIII
AflIII from New England Biolabs
Be the first to write a review!
Citations:
(9)
Supplier Page
Supplier Page from
New England Biolabs for
AflIII
Get Pricing
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
AflIII
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0541L
Concentration
5 u/µL
Quantity
1250 units
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-20 C
Sequence
A/C(AG)(CT)GT
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (9)
(1)
Ciliary Proteins Repurposed by the Synaptic Ribbon: Trafficking Myristoylated Proteins at Rod Photoreceptor Synapses
International journal of molecular sciences
June 27, 2022
Suiwal S, Dembla M, Schwarz K, Katiyar R, Jung M, Carius Y, Maxeiner S, Lauterbach MA, Lancaster CRD, Schmitz F.
restriction analysis of plasmid DNA for the silent restriction site
AflIII
(
NEB
, Frankfurt, Germany,
R0541S
) present in the point-mutated sequences. Positive
clones
were further verified by
Sanger sequencing
.
Cloning
(2)
Germinal Center B Cells Replace Their Antigen Receptors in Dark Zones and Fail Light Zone Entry when Immunoglobulin Gene Mutations are Damaging
Immunity
September 18, 2018
Stewart I, Radtke D, Phillips B, McGowan SJ, Bannard O.
214-14
Q5 High Fidelity DNA polymerase
,
New England Biolabs
,Cat#
M0491L
AFlIII
,
New England Biolabs
,Cat#
R0541S
NsiI-HF
,
New England Biolabs
,Cat#
R3127S
,, Critical commercial
assays
,Critical commercial
assays
,Critical
(3)
Designing and overproducing a tandem epitope of gp350/220 that shows a potential to become an EBV vaccine
Heliyon
March 1, 2018
Widodo, Veronica Margarecaesha Anyndita N, Dluha N, Rifa'i M, Himmah K, Wahyuningsih MD.
results were checked for validity based on the excision of the restriction enzyme (
AflIII
;
NEB
Cat No.
R0541S
). Following, the
E. coli
strain
BL21 was transformed using a newly constructed plasmid. Briefly, the
(4)
Extraction of amplifiable DNA from embalmed human cadaver tissue
BMC Research Notes
December 13, 2017
Lindsay Gielda, Stefanie Rigg
polymerase (
Promega
cat#
M7132
). Amplified DNA was digested with
HaeII
and
AflIII
(
NEB
cat#
R0107S
and
R0541S
) for 2 h at 37 °C and resolved on a 4% agarose gel. Samples were classified based on size; 145, 168,
(5)
Targeting a Single Alternative Polyadenylation Site Coordinately Blocks Expression of Androgen Receptor mRNA Splice Variants in Prostate Cancer
Cancer Research
October 1, 2017
Jamie L. Van Etten, Michael Nyquist, Yingming Li, Rendong Yang, Yeung Ho, Rachel Johnson, Olivia Ondigi, Daniel F. Voytas, Christine Henzler, Scott M. Dehm
with Qiaquick PCR purification kit (
28104
Qiagen
, Valencia, CA) and digested with 10 units of Afl III (
R0541S
New England Biolabs
, Ipswich, MA) for 2 hours. Restriction analysis was performed via
agarose gel electrophoresis
. siRNA
(6)
Light-Dependent Regulation of Sleep and Wake States by Prokineticin 2 in Zebrafish
Neuron
July 5, 2017
Shijia Chen, Sabine Reichert, Chanpreet Singh, Grigorios Oikonomou, Jason Rihel, David A. Prober
5′-GGCGTTGGTAATTGCGTATT-3′ and 5′-TATCAGCCACCAGCACTCTG-3′.
PCR
products were digested with
AflIII
(
R0541
,
New England Biolabs
Inc.). Mutant (527 bp) and WT (218 bp + 316 bp) bands were distinguished by running
(7)
Erratum to: ‘The Memory Aid study: protocol for a randomized controlled clinical trial evaluating the effect of computer-based working memory training in elderly patients with mild cognitive impairment (MCI)’
Trials
December 1, 2016
Marianne M. Flak, Susanne S. Hernes, Linda Chang, Thomas Ernst, Vanessa Douet, Jon Skranes, Gro C. C. Løhaugen
MA) for 2 hours at 37 °C, and by
HaeII
(
R0107S
,
New England Biolabs
, Beverly, MA, USA) and
AflIII
(
R0541S
,
New England Biolabs
, Beverly, MA, USA) overnight at 37 °C. The digested
PCR
products will then be analyzed
(8)
IMAGEtags: Quantifying mRNA Transcription in Real Time with Multiaptamer Reporters
Visualizing RNA Dynamics in the Cell
June 1, 2016
Judhajeet Ray, Ilchung Shin, Muslum Ilgu, Lee Bendickson, Vinayak Gupta, George A. Kraus, Marit Nilsen-Hamilton
synthetic oligonucleotides Digest pZErO-2 vector with
AflIII
(
New England Biolabs
-NEB, Ipswich, MA, Cat#
R0541S
) and
ApoI
(
NEB
,
R0566S
) restriction enzymes and isolate the 1854 base pair fragment using
gel electrophoresis
(9)
Amplification refractory mutation system polymerase chain reaction versus optimized polymerase chain reaction restriction-fragment length polymorphism for apolipoprotein E genotyping of majorly depressed patients
Molecular Medicine Reports
November 1, 2015
HONGMIN YOU et al.
72˚C for 15 min. The
AflIII
digestion mixture contained 10 µl
PCR
products and five units of
AflIII
(
R0541L
;
New England Biolabs
, Ipswich, WI, USA) in the buffer supplied by the manufacturer (
NEB
3 buffer). Similarly,
<<
<
>
>>
Reviews
Be the First to Write a Review!
AflIII from New England Biolabs
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
Back To Top